home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410o.zip
/
M94A2713.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
2KB
|
36 lines
Document 2713
DOCN M94A2713
TI Differentiation of HIV-1 infection cross reacting with HIV-2 from real
HIV-1 and HIV-2 double infection by serological absorption test.
DT 9412
AU Hayashi T; Kondo M; Saito T; Hayami M; Nishioka K; Imai M; Kanagawa
Prefectural Institute Of Health, Japan.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):241 (abstract no. PB0394). Unique
Identifier : AIDSLINE ICA10/94369862
AB OBJECTIVES: Some samples of sera from HIV-1 infected cases showed
serological cross reaction with HIV-2 causing confusion of
serodiagnosis. Therefore we tried to differentiate these cases from real
double infection with both viruses. METHODS: Sera were tested by
agglutination of HIV-1 and HIV-2 gelatin particle (PA), western blot,
pepti LAV for HIV-1 and HIV-2 and cross absorption with HIV-1 and HIV-2.
HIV progenomes were tested by specific primers of HIV-1 and HIV-2.
RESULTS: We selected two HIV infected sera in Japan strongly positive by
PA, western blot and pepti LAV for both HIV-1 and HIV-2. HIV-1 absorbed
off both HIV-1 and HIV-2 reactivity but HIV-2 could absorb HIV-2
reactivity but not HIV-1. Double infected West-African serum with HIV-1
and HIV-2 showed only corresponding reactivity which was absorbed off
with corresponding antigen. These results were also confirmed by type
specific PCR test. CONCLUSIONS: HIV-1 infection cross reacting with
HIV-2 was differentiated from real HIV-1 and HIV-2 double infection by
our serological cross absorption test, which is proven to be highly
specific and useful for serodiagnosis.
DE Absorption *AIDS Serodiagnosis Blotting, Western Comparative Study
Cross Reactions/*IMMUNOLOGY Diagnosis, Differential Enzyme-Linked
Immunosorbent Assay Human HIV Seropositivity/*DIAGNOSIS/IMMUNOLOGY
HIV-1/*IMMUNOLOGY HIV-2/*IMMUNOLOGY Polymerase Chain Reaction
Predictive Value of Tests MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).